Follicular fluid composition in relation to follicular size in pregnant and non-pregnant dromedary camels (Camelus dromedaries)
نویسندگان
چکیده
The aim of this study was to evaluate the effects of the reproductive status of female dromedary camels (pregnant vs. non-pregnant) on the chemical composition, hormonal profile and antioxidant capacity of follicular fluid collected from different sized ovarian follicles during the breeding season. One hundred ovaries were collected at slaughterhouse from fifty female dromedary camels. The ovaries were collected in pairs from each animal and allocated into two groups according to the reproductive status of the females; 25 pairs were obtained from pregnant females and 25 pairs were obtained from non-pregnant animals. The follicles on each ovary were categorized according to their diameter into three categories; small (1-3 mm), medium (4-9 mm) and large (10-20 mm). Follicular fluid (FF) aspirated from each follicle category from each pair of ovaries was analyzed. The results showed that the average number of follicles per ovary was greater (P < 0.05) in the ovaries obtained from nonpregnant females compared to those collected from pregnant ones (6.4 ± 1.2 vs. 3.6 ± 0.9, respectively). Progesterone concentrations were significantly higher in the follicular fluid collected from all follicle categories in pregnant animals than those obtained from nonpregnant animals. Glucose concentrations were higher (P < 0.05) in the follicular fluid collected from large follicles in the non-pregnant group (64.9 ± 6.1 mg/dl) than those obtained from the same follicle category in the pregnant ovaries (45.4 ± 4.0 mg/dl). Concentrations of malondialdehyde (MDA) were higher (P < 0.05) in the FF collected from small, medium and large follicles in pregnant ovaries than non-pregnant ones. In conclusion, these data indicate that FF composition differs according to the reproductive status of the female. In pregnant camels, the presence of the corpus luteum on the ovaries could play an important role not only in the process of follicle growth and development, but also in the concentrations of biochemical metabolites and hormonal profiles in the FF of
منابع مشابه
Elevated levels of blood urea nitrogen and creatinine in the last trimester of pregnancy of dromedary camels (Camelus dromedarius)
Background: The knowledge of blood parameters is important for assessing the physiological status and health of animals. OBJECTIVES: This study was conducted to determine the effect of heavy pregnancy on some serum indices of dromedary camels. METHODS: Twenty clinically healthy female dromedary camels aged between 4-5 years were divided into two equal groups: I: pregnant camels in their last tr...
متن کاملFine Structures of the Oocyte in Relation to Serum, Follicular Fluid Steroid Hormones and IGF-I in the Ovulatory-Sized Follicles in One-Humped Camel (Camelus dromedarius)
BACKGROUND The following study was carried out to determine the ultrastructural features of the oocyte of the ovulatory-sized follicles in relation to concentrations of steroids and IGF-I in the Follicular Fluid (FF) and serum in the dromedary camel. METHODS CAMEL FOLLICLES WITH A CLEAR AND HEALTHY APPEARANCE WERE CATEGORIZED INTO THREE CLASSES: follicles 10 to 13.9, 14-17.9 and 18-30 mm diam...
متن کاملA Pathological Abattoir Survey of the Reproductive Tracts of non Pregnant Camels (Camelus dromedaries) in Iraq
An abattoir survey of the female reproductive tracts of 80 camels was conducted to evaluate the gross and microscopic abnormalities. An incidence of (32.5%) of disorders was recorded from 26 female. 16 camels (20%) had uterine lesions and 10 camels (12.5%) had ovarian lesions. Salpingitis occurred in three camels (3.75%) that also had uterine inflammations. Uterine inflammations were 12 cases (...
متن کاملSequencing and Bioinformatics Analysis of Kappa-Casein Exon 4 Gene in Iranian Bacterianus and Dromedaries Camels
Kappa-casein, as a major protein component in mammalian milk, plays an essential role in formation and stabilization milk micelles and preventing them from aggregating and therefore, helping to keep calcium phosphate in solution and transfer of calcium and phosphors from animal milk to consumers. Therefore, the objective of the current study was to investigate genetic and phylogenetic analysis ...
متن کاملOVARIAN STEROID CONCENTRATIONS AND FOLLICLE DEVELOPMENT IN THE FEMALE CAMEL (Camelus dromedarius)
The main objective of the present study was to investigate the changes in ovarian oestradiol and testosterone concentrations as follicles develop in the camel. Ovaries were collected from 15 non-pregnant females from a slaughter house. All follicles ;::::4.0 mm diameter were dissected from each ovary, follicle size was recorded and follicular fluid was aspirated from each follicle and analyzed ...
متن کامل